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bio rad iscript tm gdna clear cdna synthesis kit  (Bio-Rad)


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    Bio-Rad bio rad iscript tm gdna clear cdna synthesis kit
    Bio Rad Iscript Tm Gdna Clear Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 49594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+tm+gdna+clear+cdna+synthesis+kit/iScript+cDNA+Synthesis+Kit/bio_rxiv__64898__2026__05__03__722480-126-10-10
    Average 99 stars, based on 49594 article reviews
    bio rad iscript tm gdna clear cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Generation and validation of an aryl hydrocarbon receptor knockout human embryonic stem cell line
    Article Snippet: mRNA was isolated using the RNeasy Micro kit (Qiagen, #74004) as per the manufacturer’s instructions. .. DNase treatment was performed prior to cDNA synthesis with the iScript TM gDNA Clear cDNA Synthesis Kit (Biorad, #1725035). .. RT-qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad, #1725271) and run on a Vii7 instrument (Applied Biosystems) or a CFX384 instrument (Biorad).

    Article Title: Long-read RNA sequencing identifies isoform switching of a novel long non-coding RNA that promotes somatic cell reprogramming
    Article Snippet: RNA was quantified using a NanoDrop TM 2000 Spectrophotometer (ThermoFisher #ND-2000C) and then DNase-treated using DNaseI (ThermoFisher Scientific #EN0521) according to the manufacturer’s instructions. .. 300ng of DNase-treated and non-treated RNA were loaded onto a 1% Agarose A (Bio Basic #D0012) gel to verify the RNA integrity and purity. cDNA was synthesized from 500ng of RNA by oligo-dT and random priming with iScript TM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035), according to the manufacturer’s instructions. cDNA was diluted ten times and qPCRs were performed using SYBR TM Select Master Mix (ThermoFisher Scientific #4472908) on a CFX384 Touch real-time PCR detection system (Bio-Rad). .. Raw data was retrieved using the Bio-Rad CFX Maestro (v5.3.022.1030) software.

    Article Title: Eight Triplex-Binding Molecules from Four Chemical Classes Broadly Recognize the MALAT1 Triple Helix
    Article Snippet: .. DNase treatment and cDNA synthesis were performed using the iScript TM gDNA Clear cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) per the manufacturer’s instructions. .. Previously published primer pairs were used for premature and mature MALAT1 [ ], MENβ [ ], TUG1 [ ], 18s rRNA [ ], β-actin [ ], U6 snRNA [ ], and glyceraldyhde-3-phosphare dehydrogenase (GAPDH) [ ] ( ) (Sigma-Aldrich, St. Louis, MO, USA).

    Article Title: Crosstalk between the aryl hydrocarbon receptor and hypoxia-inducible factor 1α pathways in human islet models
    Article Snippet: Immediately following treatment, SC-islets or human islets were transferred to buffer RLT (#79216, Qiagen, Hilden, Germany) and stored at −80°C. mRNA was isolated using RNeasy Micro kit (#74004, Qiagen, Hilden, Germany) as per the manufacturer’s instructions. .. DNase treatment was performed prior to cDNA synthesis with the iScript TM gDNA Clear cDNA Synthesis Kit (#1725035, Bio-Rad, Mississauga, ON, Canada). .. Real time (RT) qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, #1725271) and run on a CFX394 (Bio-Rad).

    Article Title: Molecular and functional MRI enables detection of cardiac fibrosis and evaluation of treatment response after chordin-like 1 gene therapy in myocardial infarction
    Article Snippet: The tissue was homogenized and total RNA was extracted using the miRNeasy ® Mini Kit (1038703, Qiagen, Germany) according to manufacturers' instructions. .. To remove genomic and vector DNA traces, RNA was treated with DNase I (Roche) and then reverse transcribed using the iScript TM gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad, USA) following the manufacturer's protocol. ..

    Article Title: Annexin A1 as a key modulator of lung inflammation during coronavirus infections
    Article Snippet: Lung tissues were macerated using a lysis buffer of the kit, and eluted RNA was quantified using a spectrophotometer (NanoDrop TM , Thermo Scientific). .. The cDNA was synthesized with 500ng of total RNA using the iScript TM gDNA Clear cDNA Synthesis Kit (BIO-RAD) following manufacturer’s instructions. ..

    Article Title: Annexin A1 as a key modulator of lung inflammation during coronavirus infections
    Article Snippet: Lung tissues were macerated using a lysis buffer of the kit, and eluted RNA was quantified using a spectrophotometer (NanoDrop TM , Thermo Scientific). .. The cDNA was synthesized with 500 ng of total RNA using the iScript TM gDNA Clear cDNA Synthesis Kit (BIO-RAD) following manufacturer’s instructions. ..

    Synthesized:

    Article Title: Long-read RNA sequencing identifies isoform switching of a novel long non-coding RNA that promotes somatic cell reprogramming
    Article Snippet: RNA was quantified using a NanoDrop TM 2000 Spectrophotometer (ThermoFisher #ND-2000C) and then DNase-treated using DNaseI (ThermoFisher Scientific #EN0521) according to the manufacturer’s instructions. .. 300ng of DNase-treated and non-treated RNA were loaded onto a 1% Agarose A (Bio Basic #D0012) gel to verify the RNA integrity and purity. cDNA was synthesized from 500ng of RNA by oligo-dT and random priming with iScript TM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035), according to the manufacturer’s instructions. cDNA was diluted ten times and qPCRs were performed using SYBR TM Select Master Mix (ThermoFisher Scientific #4472908) on a CFX384 Touch real-time PCR detection system (Bio-Rad). .. Raw data was retrieved using the Bio-Rad CFX Maestro (v5.3.022.1030) software.

    Article Title: Effects of organic and inorganic contaminants and their mixtures on metabolic health and gene expression in developmentally exposed zebrafish
    Article Snippet: The RNA was quantified with a Nanodrop (ThermoFisher) and diluted for the following steps. .. The gDNA was removed and cDNA was synthesized, using the iScript TM gDNA Clear cDNA Synthesis kit (Bio-Rad). .. Gene expression was measured using the SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad).

    Article Title: Annexin A1 as a key modulator of lung inflammation during coronavirus infections
    Article Snippet: Lung tissues were macerated using a lysis buffer of the kit, and eluted RNA was quantified using a spectrophotometer (NanoDrop TM , Thermo Scientific). .. The cDNA was synthesized with 500ng of total RNA using the iScript TM gDNA Clear cDNA Synthesis Kit (BIO-RAD) following manufacturer’s instructions. ..

    Article Title: Annexin A1 as a key modulator of lung inflammation during coronavirus infections
    Article Snippet: Lung tissues were macerated using a lysis buffer of the kit, and eluted RNA was quantified using a spectrophotometer (NanoDrop TM , Thermo Scientific). .. The cDNA was synthesized with 500 ng of total RNA using the iScript TM gDNA Clear cDNA Synthesis Kit (BIO-RAD) following manufacturer’s instructions. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Long-read RNA sequencing identifies isoform switching of a novel long non-coding RNA that promotes somatic cell reprogramming
    Article Snippet: RNA was quantified using a NanoDrop TM 2000 Spectrophotometer (ThermoFisher #ND-2000C) and then DNase-treated using DNaseI (ThermoFisher Scientific #EN0521) according to the manufacturer’s instructions. .. 300ng of DNase-treated and non-treated RNA were loaded onto a 1% Agarose A (Bio Basic #D0012) gel to verify the RNA integrity and purity. cDNA was synthesized from 500ng of RNA by oligo-dT and random priming with iScript TM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035), according to the manufacturer’s instructions. cDNA was diluted ten times and qPCRs were performed using SYBR TM Select Master Mix (ThermoFisher Scientific #4472908) on a CFX384 Touch real-time PCR detection system (Bio-Rad). .. Raw data was retrieved using the Bio-Rad CFX Maestro (v5.3.022.1030) software.

    Plasmid Preparation:

    Article Title: Molecular and functional MRI enables detection of cardiac fibrosis and evaluation of treatment response after chordin-like 1 gene therapy in myocardial infarction
    Article Snippet: The tissue was homogenized and total RNA was extracted using the miRNeasy ® Mini Kit (1038703, Qiagen, Germany) according to manufacturers' instructions. .. To remove genomic and vector DNA traces, RNA was treated with DNase I (Roche) and then reverse transcribed using the iScript TM gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad, USA) following the manufacturer's protocol. ..

    Reverse Transcription:

    Article Title: Molecular and functional MRI enables detection of cardiac fibrosis and evaluation of treatment response after chordin-like 1 gene therapy in myocardial infarction
    Article Snippet: The tissue was homogenized and total RNA was extracted using the miRNeasy ® Mini Kit (1038703, Qiagen, Germany) according to manufacturers' instructions. .. To remove genomic and vector DNA traces, RNA was treated with DNase I (Roche) and then reverse transcribed using the iScript TM gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad, USA) following the manufacturer's protocol. ..



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    ( A ) Experimental design. ( B ) Viral detection by qPCR assay 48 h post-infection (48 hpi). Results are expressed as relative units per 500 ng of RNA used to produce <t>cDNA</t> and are representative of two different experiments ( n = 5). ( C ) Representative images of lung sections stained by hematoxylin and eosin (mock group and 48hpi) (scale bar 200 μm and 50 μm). ( D ) Histopathological scores in lung sections ( n = 5) of BALB/c WT and AnxA1KO mice. ( E ) Vascular leakage evaluated by Evans Blue concentration assay in the lungs. Concentration of TNF, IL-6, and CXCL1 in the lungs (F, G, and H, respectively) (expressed as pg/ml of homogenized tissue supernatant) ( n = 5). ( H ) *** P <0.01 (WT vs. AnxA1KO 48hpi). ** P <0.0001 (WT vs. AnxA1KO 48hpi in B and G). n.s.: not significant. In ( B ), Mann–Whitney test was used to assess the differences between the groups. From ( D ) to ( H ), two-way ANOVA tests followed by Tukey’s multiple comparison test were used to assess the differences between the groups. Results are expressed as mean ± SEM. AnxA1, annexin A1; KO, knockout.
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    iscript gdna clear tm cdna synthesis kit  (Bio-Rad)
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    ( A ) Experimental design. ( B ) Viral detection by qPCR assay 48 h post-infection (48 hpi). Results are expressed as relative units per 500 ng of RNA used to produce <t>cDNA</t> and are representative of two different experiments ( n = 5). ( C ) Representative images of lung sections stained by hematoxylin and eosin (mock group and 48hpi) (scale bar 200 μm and 50 μm). ( D ) Histopathological scores in lung sections ( n = 5) of BALB/c WT and AnxA1KO mice. ( E ) Vascular leakage evaluated by Evans Blue concentration assay in the lungs. Concentration of TNF, IL-6, and CXCL1 in the lungs (F, G, and H, respectively) (expressed as pg/ml of homogenized tissue supernatant) ( n = 5). ( H ) *** P <0.01 (WT vs. AnxA1KO 48hpi). ** P <0.0001 (WT vs. AnxA1KO 48hpi in B and G). n.s.: not significant. In ( B ), Mann–Whitney test was used to assess the differences between the groups. From ( D ) to ( H ), two-way ANOVA tests followed by Tukey’s multiple comparison test were used to assess the differences between the groups. Results are expressed as mean ± SEM. AnxA1, annexin A1; KO, knockout.
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    Image Search Results


    ( A ) Experimental design. ( B ) Viral detection by qPCR assay 48 h post-infection (48 hpi). Results are expressed as relative units per 500 ng of RNA used to produce cDNA and are representative of two different experiments ( n = 5). ( C ) Representative images of lung sections stained by hematoxylin and eosin (mock group and 48hpi) (scale bar 200 μm and 50 μm). ( D ) Histopathological scores in lung sections ( n = 5) of BALB/c WT and AnxA1KO mice. ( E ) Vascular leakage evaluated by Evans Blue concentration assay in the lungs. Concentration of TNF, IL-6, and CXCL1 in the lungs (F, G, and H, respectively) (expressed as pg/ml of homogenized tissue supernatant) ( n = 5). ( H ) *** P <0.01 (WT vs. AnxA1KO 48hpi). ** P <0.0001 (WT vs. AnxA1KO 48hpi in B and G). n.s.: not significant. In ( B ), Mann–Whitney test was used to assess the differences between the groups. From ( D ) to ( H ), two-way ANOVA tests followed by Tukey’s multiple comparison test were used to assess the differences between the groups. Results are expressed as mean ± SEM. AnxA1, annexin A1; KO, knockout.

    Journal: Clinical Science (London, England : 1979)

    Article Title: Annexin A1 as a key modulator of lung inflammation during coronavirus infections

    doi: 10.1042/CS20255801

    Figure Lengend Snippet: ( A ) Experimental design. ( B ) Viral detection by qPCR assay 48 h post-infection (48 hpi). Results are expressed as relative units per 500 ng of RNA used to produce cDNA and are representative of two different experiments ( n = 5). ( C ) Representative images of lung sections stained by hematoxylin and eosin (mock group and 48hpi) (scale bar 200 μm and 50 μm). ( D ) Histopathological scores in lung sections ( n = 5) of BALB/c WT and AnxA1KO mice. ( E ) Vascular leakage evaluated by Evans Blue concentration assay in the lungs. Concentration of TNF, IL-6, and CXCL1 in the lungs (F, G, and H, respectively) (expressed as pg/ml of homogenized tissue supernatant) ( n = 5). ( H ) *** P <0.01 (WT vs. AnxA1KO 48hpi). ** P <0.0001 (WT vs. AnxA1KO 48hpi in B and G). n.s.: not significant. In ( B ), Mann–Whitney test was used to assess the differences between the groups. From ( D ) to ( H ), two-way ANOVA tests followed by Tukey’s multiple comparison test were used to assess the differences between the groups. Results are expressed as mean ± SEM. AnxA1, annexin A1; KO, knockout.

    Article Snippet: The cDNA was synthesized with 500 ng of total RNA using the iScript TM gDNA Clear cDNA Synthesis Kit (BIO-RAD) following manufacturer’s instructions.

    Techniques: Infection, Staining, Concentration Assay, MANN-WHITNEY, Comparison, Knock-Out